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Later that year, a study compared the DNA sequences using 127,000 single-nucleotide polymorphisms (mutations) of wolves and coyotes, but did not include red wolves and used Algonquin wolves as the representative eastern wolf, not wolves from the western Great Lakes states (usually referred to as Great Lakes wolves). The study indicated that Algonquin wolves were a distinct genomic cluster, even distinct from the Great Lakes states’ wolves, which it found were actually hybrids of the gray wolf and the Algonquin wolf. The study’s results did not exclude a possibility that the Great Lakes states’ wolf (the gray wolf x eastern wolf hybrid (C. l. lycaon)) historically inhabited southern Ontario, southern Quebec and the northeastern United States alongside the Algonquin wolf, as there is evidence to suggest both inhabited those areas.

In 2016, a study of mDNA once again indicated the Eastern wolf as a coyote–wolf hybrid.

In 2018, a study looked at the y-chromosome male lineage of canines. The unexpected finding was that the one Great Lakes wolf specimen included in this study showed a high degree of genetic divergence. Previous studies propose the Great Lakes wolf to be an ancient ecotype of the gray wolf that had experienced genetic introgression from other types of gray wolves and coyotes. The study called for further research into the Y-chromosomes of coyotes and wolves to ascertain if this is where this unique genetic male lineage may have originated from.
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Wolf distribution
Wolf populations strongly declined across Europe during the 18th and 19th centuries largely due to human persecution, and by the End of World War II in Europe they had been extirpated from all of Central Europe and almost all of Northern Europe.

The extirpation of Northern Europe’s wolves first became an organized effort during the Middle Ages, and continued until the late 19th century. In England, wolf eradication was enforced by legislation, and the last wolf was killed in the early 16th century during the reign of Henry VII (reigned 1485-1509). Wolves lasted longer in Scotland, where they sheltered in vast tracts of forest, which were subsequently burned down. Wolves managed to survive in the forests of Braemar and Sutherland until 1684. The extirpation of wolves in Ireland followed a similar course, with the last wolf believed to have been killed in 1786. A wolf bounty was introduced in Sweden in 1647, after the extirpation of moose and reindeer forced wolves to feed on livestock. The Sami extirpated wolves in northern Sweden in organized drives. By 1960, few wolves remained in Sweden, because of the use of snowmobiles in hunting them, with the last specimen being killed in 1966. The gray wolf was extirpated in Denmark in 1772 and Norway’s last wolf was killed in 1973. The species was decimated in 20th century Finland, despite regular dispersals from Russia. The gray wolf was only present in the eastern and northern parts of Finland by 1900, though its numbers increased after World War II.

In Central Europe, wolves were dramatically reduced in number during the early 19th century, because of organized hunts and reductions in ungulate populations. In Bavaria, the last wolf was killed in 1847, and had disappeared from the Rhine regions by 1899. In Switzerland, wolves were extirpated in the 20th century; they are naturally coming back from Italy since the 1990s. In 1934, Nazi Germany became the first state in modern history to place the wolf under protection, though the species was already extirpated in Germany at this point. The last free-living wolf to be killed on the soil of present-day Germany before 1945 was the so-called “Tiger of Sabrodt”, which was shot near Hoyerswerda, Lusatia (then Lower Silesia) in 1904. Today, wolves have returned to the area. Wolf hunting in France was first institutionalized by Charlemagne between 800–813, when he established the louveterie, a special corps of wolf hunters. The louveterie was abolished after the French Revolution in 1789, but was reestablished in 1814. In 1883, up to 1,386 wolves were killed, with many more by poison.

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